Author : Sneha sabu, sofia khanam, and a. jothilin subitsha
Keyword : Agrobacterium tumefaciens, oryza sativa, t-dna, genetic transformation, transgene expression, transgenic rice
Subject : Microbiology
Article Type : Review article
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Abstract : Since the beginning of the twentieth century, Agrobacterium tumefaciens and associated species of Agrobacterium have been referred to as plant pathogens. However, the potentiality of Agrobacterium to displace DNA to plant cells has been harnessed for plant genetic engineering purposes for the past two decades. For more than half of the world's population, Oryza sativa (rice) is the staple food and has also been a major monocot model. Plant scientists have used techniques of incorporating DNA into plant genomes to investigate gene functions. Transformation also provides an important method for enhancing breeding for crops such as rice. To explore gene functions, plant scientists have used methods of inserting DNA into plant genomes. The study explored the various restrictions that hinder the progressive transformation of genetic rice via the Agrobacterium-mediated method and proposed potential solutions. The pinpointing of explant, a strategy for gene exchange, and development to modify incorporation, transgene articulation without affirmation to hereditary harm and determination of transformant are among the innovative difficulties affecting rice transformation. Due to its exact T-DNA handling and simple consolidation of low copy number transgenes, the Agrobacterium-mediated transformation strategy was a superior decision for creating transgenic assortments of rice. This article attempts to overview the fundamental biology associated with genetic transformation promoted by Agrobacterium, which may perhaps be beneficial to both plant biologists and microbiologists who want a deeper perception of the expression of plant proteins, the transformation of plants, and the association between plant and microbe.
Article by : Sofia Khanam
Article add date : 2021-06-11
How to cite : Sneha sabu, sofia khanam, and a. jothilin subitsha. (2021-June-11). Agrobacterium-mediated transformation in oryza sativa (rice) to improve crop yield: a review. retrieved from https://www.openacessjournal.com/abstract/761