Author : A kandan, jameel akhtar, b singh, d pal, d chand, pc agarwal and sc dubey
Keyword : Colletotrichum capsici, capsicum annuum, fungal pathogens, lamp assay
Subject : Agricultural research
Article Type : Original article (research)
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Abstract : Colletotrichum capsici is an economically important pathogen that severely affects the fruits of chilli (Capsicum annuum) plants. Loop-mediated isothermal amplification (LAMP) assay was used for accurate and sensitive detection of C. capsici. Sequences of β-tubulin gene of C. capsici were used as target template to design LAMP primers. The primers were v cted chilli seeds collected from different locations in India showed that the LAMP assay was more sensitive than the PCRbased detection of C. capsici. This LAMP assay is simple, fast and sensitive in detection of C. capsici infected field samples, tissue culture materials and for schedule screening of plant quarantine materials under exchange. In the present study, LAMP assay has been used for fast and sensitive detection of C. capsici pathogen.
Article by : Jameel Akhtar
Article add date : 2021-01-19
How to cite : A kandan, jameel akhtar, b singh, d pal, d chand, pc agarwal and sc dubey. (2021-January-19). Application of loop-mediated isothermal amplification (lamp) assay for rapid and sensitive detection of fungal pathogen, colletotrichum capsici in capsicum annuum. retrieved from https://www.openacessjournal.com/abstract/572