Author : Veysel yuksek, sedat cetin, ayse usta, ahmet ufuk komuroglu, semiha dede
Keyword : Naf, cell culture, antioxidant, oxidative state
Subject : Biochemistry
Article Type : Original article (research)
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Abstract : Objective: This study was planned to determine the effect of certain vitamin applications on antioxidant and oxidant parameters in the osteoblast cell line exposed to sodium fluoride in vitro and to evaluate the protective role of certain vitamins against possible toxic effects of fluoride. Materials and Methods: Cells were replicated in vitro conditions with regular passaging 2-3 times weekly. MTF viability test was used to determine IC50 of NaF (5000μM) and proliferative doses of vitamins (Vitamin A: 10μM, Vitamin D: 10μM, Vitamin E: 60μM, Vitamin C: 100μM) for hFOB 1.19 cells. Cells were sown in flasks as so to be 106. The study groups were identified as control, NaF, vitamins and, NaF+vitamins. After incubation for 24 hours, cells treated with trypsin were prepared by freeze/thaw method and MTT viability test, TAS, SOD, GSH, CAT, TOS, and MDA analyzes were performed on these samples. Results: In the hFOB 1.19 cell line, TAS levels decreased significantly in the NaF group (p≤0.05), but were close to the control group in NaF+vitamin groups with the exception of vitamin C. However, there was no difference between the groups in terms of GSH level and CAT and SOD activities when the control and NaF groups were compared. It was observed that TOS level increased significantly in the NaF group (p
Article by : Ebubekir Ceylan
Article add date : 2021-01-05
How to cite : Veysel yuksek, sedat cetin, ayse usta, ahmet ufuk komuroglu, semiha dede. (2021-January-05). Effect of some vitamins on antioxidant/prooxidant parameters in sodium fluoride (naf)-treated cell line (hfob 1.19). retrieved from https://www.openacessjournal.com/abstract/516