Author : Dr atif amin1 , dr muhammad umar farooq2 , alina ikram3 , hafiza sobia ramzan4
Keyword : Porphyromonas, α-amylase, pentamidine
Subject : Pathology
Article Type : Original article (research)
DOI : http://dx.doi.org/10.22192/ijarbs.2018.05.04.008
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Abstract : Purpose: Oral infections are caused by more than 600 bacterial species; among them the most influential pathogens is Porphyromonas gingivalis. In this study, we have implemented a spectrum of antimicrobial activity for these species using a cell growth inhibitor α-amylase, and the bisbenzamidine derivative, pentamidine. Methods: The in vitro inhibitory activity was investigated with the agar overlay technique. Minimal inhibitory concentrations (MIC), minimal bactericidal concentrations (MBC) were determined. After determining the MBC, we then investigate the differential gene expression by exposing the culture with MBC. Its antimicrobial properties were investigated through microscope analysis, spectrum of activity and general structure. Results: The MIC of 6, 100 ng/mL and MBC of 12, 100ng/mL were determined for α-amylase and pentamidine, respectively. Scanning electron microscopy micrograph analysis and bactericidal assay indicated an intriguing possibility that the pentamidine and α-amylase compromises the cell membrane structure of the bacterial cells, which leads to cell death. The positive results with α-amylase and pentamidine led us to explore some mechanisms of action. The expression of the genes encoding hemagglutinins, gingipains, hemin uptake loci, chromosome replication, and energy production was downregulated, while that of the genes related to iron storage and oxidative stress was upregulated by both α-amylase and pentamidine. Conclusion: This finding highlights the promising consequence and therapeutic potential of α-amylases and pentamidine. These drugs might be innovative molecules to broaden pharmacological tools for attenuation of oral infections caused by Porphyromonas gingivalis.
Article by : hafiza sobia ramzan
Article add date : 2020-11-16
How to cite : Dr atif amin1 , dr muhammad umar farooq2 , alina ikram3 , hafiza sobia ramzan4. (2020-November-16). Attenuation of porphyromonas gingivali, an oral infection pathogen by α-amylase and pentamidine. retrieved from https://www.openacessjournal.com/abstract/363