Author : Pal neha , singh n. p. and joshi m. d.
Keyword : Antibacterial activity, minimum inhibitory concentration, plant extracts, zone of inhibition.
Subject : Microbiology
Article Type : Original article (research)
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Abstract : Objectives: To calculate the antimicrobial action of medicinal plants against human pathogenic bacteria and observe Minimum Inhibitory Concentration (MIC) of extracts of plant. Methods: Rhizome of Curcuma longa, dried buds of Syzygium aromaticum, and seed of Piper nigrum and leaves of Hibiscus-rosa-sinensis, Murraya koenigiiwere collected from local market of Uttar Pradesh. The plant parts were air-dried at room temperature and crushed to powder form. The ethanolic extracts of medicinal plants were adapted by percolation process using separating funnel and tested against human pathogenic bacteria by disc diffusion method. Then, Minimum Inhibitory Concentration (MIC) of the plant extracts was decisive. Results: All plants extracts show antibacterial properties against bacteria under study. However, extract from S. aromaticum (Clove), Hibiscus-rosa-sinensis (Gulhar) and Tinospora cordifolia (Giloy) reveal most promising result against Staphylococcus aureus with zone of inhibition of 14.33mm, 12.90mm and 16.10mm respectively. Likewise, S. aromaticum (Clove), C. longa (Turmeric) and P.nigrum (Black pepper) showed good antibacterial activity against Escherichia coli with inhibition zone of 14.10mm, 12.03mm and 12.90mm respectively. S.aromaticum (Clove) and Tinospora cordifolia (Giloy) showed promising activity against E.coli with inhibition zone of 19.67mm and 10.23mm. S.aromaticum (Clove) showed good result against P.aeruginosa with inhibition zone 19.53mm. MIC values of methanolic extracts of S. aromaticum and T.cordifolia were found to be at the range of 10.80mm. Conclusion: This study keep maintained to understand the use of these plants while traditional medicine as an economic and safe alternative to treat infectious diseases.
Article by : Neha
Article add date : 2024-01-09
How to cite : Pal neha , singh n. p. and joshi m. d.. (2024-January-09). "antimicrobial activity of methanolic extract of some medicinal plants. retrieved from https://www.openacessjournal.com/abstract/1465